Juan Arguelles

University of Oviedo / Universidad de Oviedo, Physiology, Faculty Member

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Address: Oviedo, Asturias, Spain

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Sex-specific influence of maternal exposure to bisphenol A on sodium and fluid balance in response to dipsogenic challenges in rats

Appetite

Cardio-Pulmonary Resuscitation (CPR) in Children Between 5 and 8 Years Old: Psychometric Properties of Nonide Scale

Psicothema, 2021

BACKGROUND Early intervention in a cardiorespiratory arrest by a witness significantly increases ... more BACKGROUND Early intervention in a cardiorespiratory arrest by a witness significantly increases survival. That is why early training in cardiopulmonary resuscitation (CPR) may be essential. Therefore, the aim of this study is the development and validation of an instrument to assess CPR knowledge and skills for schoolchildren from 5 to 8 years old. METHOD We used a Spanish sample with 164 children aged between 5 and 8 years old. These children received a workshop called the “CPR from my school” program and their knowledge of CPR was assessed pre- and post-Workshop. We examined the scale through psychometric analyses within the framework of Classical Test Theory. RESULTS The Nonide Scale provided evidence of content validity, revealed an essentially unidimensional internal structure, as well as excellent reliability (Ω = .87). Furthermore, a significant improvement was observed in scores, before and after the CPR Workshop. CONCLUSIONS The Nonide Scale, designed to measure CPR knowle...

Short-term exposure to bisphenol A affects water and salt intakes differently in male and ovariectomised female rats

Appetite, 2018

Thirst and sodium appetite are motivational states that lead to the search for and ingestion of w... more Thirst and sodium appetite are motivational states that lead to the search for and ingestion of water and sodium, thus contributing to maintenance of hydromineral balance. Oestrogens affect fluid intake and sodium preference. Bisphenol A (BPA) is an environmental endocrine-disrupting substance with oestrogenic activity. Whether there are sex differences in the ingestive effects of BPA is unknown. Therefore, the aim of the present work was to determine the effect of short-term treatment of low doses of BPA on thirst and sodium appetite in adult male and ovariectomised Wistar rats. Rats were subcutaneously injected with either vehicle or 10, 50, 100, or 500 μg/kg/day BPA for a week. Two-bottle tests (water and 2.7% NaCl solution) were conducted in the animals' home cages with and without 24 h water deprivation. BPA reduced water and 2.7% NaCl intakes in a sex-dependent-manner. Fluid intakes displayed inverted U-shaped dose-response curves, with different dose dependencies in spont...

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Offspring’s hydromineral adaptive responses to maternal undernutrition during lactation

Journal of Developmental Origins of Health and Disease, 2015

Early development, throughout gestation and lactation, represents a period of extreme vulnerabili... more Early development, throughout gestation and lactation, represents a period of extreme vulnerability during which susceptibility to later metabolic and cardiovascular injuries increases. Maternal diet is a major determinant of the foetal and newborn developmental environment; maternal undernutrition may result in adaptive responses leading to structural and molecular alterations in various organs and tissues, such as the brain and kidney. New nephron anlages appear in the renal cortex up to postnatal day 4 and the last anlages to be formed develop into functional nephrons by postnatal day 10 in rodents. We used a model of undernutrition in rat dams that were food-restricted during the first half of the lactation period in order to study the long-term effects of maternal diet on renal development, behaviour and neural hydromineral control mechanisms. The study showed that after 40% food restriction in maternal dietary intake, the dipsogenic responses for both water and salt intake wer...

format_quoteMaternal protein restriction during lactation resulted in a significant 25% reduction in nephron number in offspring, impacting kidney development.format_quote

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Circadian urinary citrate excretion in a rat model of exercise

Life Sciences, 2017

Aims: Circadian rhythms are the approximate 24 h biological cycles that function to prepare an or... more Aims: Circadian rhythms are the approximate 24 h biological cycles that function to prepare an organism for daily environmental changes. Circadian rhythms unquestionably play critical roles in metabolic homeostasis and the exercise has emerged as a strong non-photic time cue or zeitgeber in animal models and humans. Numerous studies on the effects of exercise on the citrate synthase activity have been published. Citrate is used to assess energy production or expenditure because it is a substrate of the Krebs Cycle, a cycle for oxidative energy production. Main methods: We tested the existence of a rhythmic urinary citrate excretion in a rat model that is made to exercise at six different points during the day.

format_quoteCorrelations found between circadian rhythm disruptions and increased metabolic disease risks; exercise may mitigate this.format_quote

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[Blood pressure elevation in obese descendants of parents with essential arterial hypertension and its relation to the renin-angiotensin axis, C-reactive protein, and saline taste sensitivity]

Nefrología : publicación oficial de la Sociedad Española Nefrologia, 2006

Exercise Effects on Circadian Rhythmicity of Urine Osmolality and Diuresis in Rats

Adaptive Medicine, 2014

Sportsmen have to maintain hydration monitoring for health, but biomarkers are transient during t... more Sportsmen have to maintain hydration monitoring for health, but biomarkers are transient during the process of dehydration and rehydration. The aim of this study was to examine the circadian rhythmicity of urine osmolality and diuresis in rats at 4 h intervals, and to determine whether training exercise resulted in changes in this rhythmicity. Rats were trained progressively on a running wheel over four days. Two urine group samples were taken from each animal; the first was taken before the start of the training program and the second was taken at the completion of the last running trial. We found a characteristic circadian rhythmicity in diuresis and in urine osmolality, which was the same for pre- and postexercise patterns. However, post- exercise urine osmolality values were greater than pre-exercise values when the exercise intensity was of sufficient intensity to reduce urine production. The urine osmolality was elevated during the approximately 4 h period after termination of the exercise, and remained high up to 16 h after the termination of exercise, after which osmolality started to normalize. Thus, this study suggests that the urinary measures described herein represent a noninvasive and easy-to-use tool for the characterization of 24 h hydration status.

Changes of blood pressure responsiveness in rats exposed in utero and perinatally to a high-salt environment

Regulatory Peptides, 1996

We tested the hypothesis that the pressor responses to angiotensin II could be influenced by an e... more We tested the hypothesis that the pressor responses to angiotensin II could be influenced by an early salt exposure. Twenty-five adult female rats were pseudorandomly divided in two groups. Twelve animals underwent a partial ligature of their abdominal aorta (PAL). Once polydipsia and sodium appetite developed, these rats were mated. The other group (13 rats) was sham-operated (Sham) and mated. Throughout pregnancy and lactation, water and 2.7% NaCI solution intakes differed between the two groups of mother rats. PAL offspring (PAL-O; n = 14), and Sham-operated offspring (Sh-O; n = 10), were maintained on a solid diet containing 1% NaC1, tap water and a 2.7% NaC1 solution. At 90 days of age, pressor responsiveness to intravenous angiotensin II (50, 100 and 200 ng) was assessed in anesthetized rats. The pressor responses to 50 and 200 ng angiotensin II were significantly greater in PAL-O rats than in Sh-O rats. These results support the hypothesis of a modulation of cardiovascular responsiveness or its underlying mechanisms by an early high salt environment.

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Effect of Castration and Gonadal Hormones on Insulin-Induced Drinking

Pharmacology Biochemistry and Behavior, 1998

Female Wistar rats in any of the estrual phases have been shown to drink significantly more water... more Female Wistar rats in any of the estrual phases have been shown to drink significantly more water than males (p &lt; 0.05), after a single I.P. insulin injection (5 U/kg b.wt.). Sexual differences in insulin-induced drinking persisted after castration when it was made in adult rats (4.6 +/- 1.2 ml/2 h, males = 8; vs. 13.0 +/- 3.1 ml/2 h, females = 8; p &lt; 0.05). On the other hand, when animals were castrated before puberty or when newborn, sexual differences in insulin-induced drinking disappeared. Hence, insulin-induced drinking seems to be a sex-dependent phenomenon that differentiates just before or during puberty since it is abolished by castration prior to sexual maturation. Sex hormone administration in male and female rats castrated at different ages showed a variety of actions on insulin-induced drinking. A pattern emerged showing that androgenized (testosterone treated) rats drank usually less in response to insulin than estrogen-treated rats (independent of their genetic sex). According to the above results, we can conclude that insulin-induced drinking is a phenomenon sensible to gonadal hormones, both by conditioning the differentiation of some physiological structure or mechanisms that underlay drinking behavior in that paradigm and by a direct action on these or other related mechanisms.

Epigenetic Programming of Water Drinking and Sodium Intake

Handbook of Nutrition, Diet, and Epigenetics, 2019

Water is the main element of the human body, representing about 70% of our body mass. This water ... more

format_quoteThree-day-old rat pups show a specific appetite for sodium, separate from thirst, due to ANG II activation.format_quote

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Chronic exposure to low doses of bisphenol A alters hydromineral responses in rats

Appetite, 2021

Bisphenol A (BPA) is a chemical commonly used in the industrial sectors, hence humans are exposed... more Bisphenol A (BPA) is a chemical commonly used in the industrial sectors, hence humans are exposed to the compound repetitively. BPA is an endocrine disruptor and has been anticipated to interfere on chemical estrogen receptor functions and other nuclear hormone receptors. Estrogens are steroid hormones that, in addition to their neuroendocrine roles, affect water and salt intakes in numerous species, including humans and rodents. Changes in the hydrosaline balance produce compensatory behavioral and physiological responses, which serve to preserve or restore osmolarity and blood volume to optimal levels, thus preventing cardiovascular disease. The aim of the present work was to determine for first time the effect of long-term and low-dose BPA treatment on thirst and sodium appetite. Wistar rats were exposed to BPA via drinking water to mimic the most likely route of human exposure, and different dipsogenic and natriorexigenic stimuli were assessed. The BPA-treated rats tend to drink less water that control rats following 24-h fluid restriction, but there was no statistically significant decrease. Perhaps the BPA dose does not have enough estrogenic potency to affect water intake. In the extracellular fluid depletion test, the control rats significantly increased 2.7% NaCl solution intake on repeated testing, showing sodium appetite sensitization, i.e. the capacity to enhance sodium intake produced by stimulus repetition; whereas BPA-treated rats did not. In this study, fluid and electrolyte balance in BPA-treated rats is generally adequate but impaired in osmotic challenges, for example by sodium depletion. Thus, neuroendocrine systems involved in maintaining body fluid and electrolyte homeostasis were altered in BPA-treated rats.

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The effects of experimental gestational hypertension on maternal blood pressure and fluid intake and pre-weanling hypothalamic neuronal activity

Appetite, Sep 12, 2017

To examine the fetal programming effects of maternal hypertension, natriophilia and hyperreninemi... more To examine the fetal programming effects of maternal hypertension, natriophilia and hyperreninemia [experimentally induced in rats by partial inter-renal aortic ligature (PAL) prior to mating] fos immunoreactivity was studied in 6-day-old offspring of PAL and control mothers. The purposes of the present set of experiments were twofold. The first was to characterize the effects of PAL on the mother's arterial blood pressure and intake of salt (1.8% NaCl solution) and water over the course of gestation. Second, was to study the pattern of neuronal activation in key brain areas of 6-day-old offspring treated with the dipsogen isoproterenol that were from PAL and control mothers. Beta-adrenergic receptor agonist-treated pups allowed the determination whether there were neuroanatomical correlates within the neural substrates controlling thirst and the enhanced water intake evidenced by the isoproterenol treated pups of PAL mothers. Hydromineral ingestive behavior along with blood pre...

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Ingestive behavior in rat pups is modified by maternal sodium depletion

Psicothema, 2012

Developmental programming by maternal stress during pregnancy is found to influence behavioral de... more Developmental programming by maternal stress during pregnancy is found to influence behavioral development in the offspring. The main objective of this study was to investigate the effect of maternal sodium depletion in rats during pregnancy on the development of thirst mechanisms in the offspring. Pregnant rats underwent 3 episodes of saline depletion, induced by injecting sc 10 mg of Furosemide in saline (0.5 ml). The treatment, given on the 14th, 17th and 20th days post-conception, is thought to induce acute sodium depletion on dams. The offspring were tested for their drinking responses to Isoproterenol (500 µg/kg sc). In accordance to the known sequence of ontogenic development of drinking mechanisms, all groups of pups drunk after being stimulated with Isoproterenol at 6 days of age. The offspring from Furosemide-treated dams drank significantly less than the control group after Isoproterenol (p<0.001). Nevertheless, basal intake (water drunk after vehicle-saline only) was ...

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Developmental programing of thirst and sodium appetite

Neuroscience & Biobehavioral Reviews, 2015

Highlights  We review here the ontogeny of thirst and sodium appetite  Thirst and sodium appe... more Highlights  We review here the ontogeny of thirst and sodium appetite  Thirst and sodium appetite are programmed by the developmental environment  Hydromineral/neuroendocrine disorders and lifestyle are the main programming factors  Several neuroendocrine systems are epigenetically regulated and potentially involved

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Williams syndrome

Oral Surgery, Oral Medicine, Oral Pathology, 1992

Williams syndrome is a rare anomaly consisting of idiopathic hypercalcemia that is normally accom... more Williams syndrome is a rare anomaly consisting of idiopathic hypercalcemia that is normally accompanied by aortic stenosis, moderate mental retardation, and a characteristic elfin face. Because persons with this syndrome have severe dental abnormalities, it is in the dental or orthodontic clinic that the disease can eventually be detected. A unique case of this type is reported.

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Increased salt preference in adult offspring raised by mother rats consuming excessive amounts of salt and water

Regulatory Peptides, 1996

This study was designed to evaluate the effects of an early high-salt environment on the maternal... more This study was designed to evaluate the effects of an early high-salt environment on the maternal and the young offspring physiology and on the adult offspring sodium appetite. Twenty-five adult female Wistar rats were pseudorandomly divided into two groups. Twelve animals underwent a partial ligature of their abdominal aorta (PAL). Once polydipsia and sodium appetite (tested by measuring water and a 2.7% NaC1 intakes) developed, they were mated. The other 13 rats (SHAM) were sham-operated and also mated. Throughout pregnancy and lactation, water and salt intake of PAL rats was consistently and significantly higher than that of the Sham. On gestation day 20, amniotic fluid and maternal plasma sodium concentration of PAL and Sham rats did not differ. Sodium concentration in the milk of the lactating PAL group was elevated (P < 0.05) on day 20 after delivery. At 0, 10 and 21 days of age, plasma sodium concentration of PAL offspring (PAL-O) and Sham offspring (Sh-O) were not significantly different. At 90 days of age, the salt preference of PAL-O rats was greater than that of Sh-O rats after 7 days of sodium deprivation (P < 0.01).

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Maternal RAS influence on the ontogeny of thirst

Physiology & Behavior, 2007

Perillan, C., Costales, M., Vijande, M., and J. Arguelles. Maternal RAS influence on the ontogeny... more Perillan, C., Costales, M., Vijande, M., and J. Arguelles. Maternal RAS influence on the ontogeny of thirst. Physiol Behav XX (X) 000-000, 2006. The main objective of this study was to investigate the effect of an altered ambiance in utero, on the development of thirst mechanisms in the offspring. Female rats underwent a partial ligature of the aorta (PAL), which induces an intrinsic activation of the renin angiotensin system (RAS), thirst and sodium appetite. A second group of female rats was treated with desoxycorticosterone (DOCA) which depresses the RAS. The offspring of these two groups were tested for their responses to several thirst stimuli at 2, 4 and 6 days of age. The offspring from PAL mothers responded like their controls to cellular dehydration (NaCl hypertonic injection) at 2 days of age, and also did to extracellular dehydration by polyethyleneglycol at 4 days. Nevertheless, they responded more to isoproterenol at 6 days of age in comparison to their control group. The offspring from DOCA treated mothers did not show statistically significant responses (in comparison with vehicle injected pups) to hypertonic NaCl at two days nor to polyethyleneglycol at four days. Water intake at 6 days of age after isoproterenol administration in DOCA was statistically enhanced, but not differently from the response obtained from pseudo-DOCA treated pups. In particular, rats developed in a hypereninemic ambiance (O-PAL) during gestation, responded with higher water intake when treated with a strong RAS and thirst activator (isoproterenol) but responded normally to a more gentle and complex stimulus (PG). Therefore it seems that in utero conditions can determine the chronology and intensity of thirst responses in offspring.

Thirst changes in offspring of hyperreninemic rat dams

Pharmacology Biochemistry and Behavior, 2004

The aim of this study was to investigate the changes in thirst dependence on the renin-angiotensi... more The aim of this study was to investigate the changes in thirst dependence on the renin-angiotensin system (RAS), in offspring of hyperreninemic, hyperdipsic, and natriophilic rat dams. Female rats underwent a partial aortic ligature between the renal arteries (PAL) or were sham-operated (SHAM). At 6 days of age, offspring of PAL (O-PAL) and SHAM (O-SHAM) dams were injected with isoproterenol (subcutaneously, 500 microg/kg body weight) or vehicle. Pretreatment with captopril (intraperitoneally, 50 mg/kg) on isoproterenol-induced thirst was also studied. Plasma renin activity in dams and hematocrit and osmolality in pups were measured. O-PAL had a greater water intake than O-SHAM. However, they responded similarly to isoproterenol or isoproterenol with captopril pretreatment. Only minor differences in hematocrit and osmolality were found between O-SHAM and O-PAL rats after isoproterenol or vehicle treatment. Beta-adrenergic or angiotensinergic responsivity seems not to be altered in offspring of hyperrenimic, hyperdipsic, and natriophilic dams. Nevertheless, other thirst responses of offspring may be critically dependent upon uterine conditions.

Corrigendum to “Progressive training effects on neuronal hypothalamic activation in the rat” [Neurosci. Lett. 517 (2012) 113–117]

Neuroscience Letters, 2014

Partial aortic ligature induces selective long-term c-fos like immunoreactivity in the organum vasculosum of the lamina terminalis, medial preoptic area and choroid plexus in the rat

Neuroscience Letters, 2001

Partial aortic ligature causes an increase in water and sodium intake. Circumventricular brain re... more Partial aortic ligature causes an increase in water and sodium intake. Circumventricular brain regions are known to be involved in the regulation of these processes. In this work we use c-fos-like immunoreactivity to detect active areas involved in the long-term control of increased water and sodium intake due to partial aortic ligature. A signi®cant increase in water intake was found on the ®rst day after the induction, while natriophilia was observed on the fourth day. c-fos-like immunoreactivity was found selectively in the subfornical organ, the organum vasculosum of the lamina terminalis, the medial preoptic area, and the choroid plexus of the third ventricle. Present results provide further evidence for the involvement of circumventricular organs and the preoptic area in the regulation of hydromineral balance. Moreover, they suggest a maintained and long-term regulation of sodium intake by these same brain areas.

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Juan Arguelles

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